MEK1 anticorps (PE)

N° du produit ABIN1176908

L’anticorps Souris Monoclonal anti-MEK1 a été validé pour ICS. Il convient pour détecter MEK1 dans des échantillons de Humain, Souris, Rat, Chien et Poulet. Il y a 3+ publications disponibles.

Aperçu rapide pour MEK1 anticorps (PE) (ABIN1176908)

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MEK1 est conjugé à/à la PE

Application: Intracellular Staining (ICS)

Clone: 25-MEK1

Détail du produit anti-MEK1 anticorps (PE)

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human MEK1 Recombinant Protein

Isotype: IgG2a

Information d'application

Indications d'application: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-MEK1 (PE) (ABIN1176908)

Gu, Fujibayashi, Yamada, Sekiguchi: "Laminin-10/11 and fibronectin differentially prevent apoptosis induced by serum removal via phosphatidylinositol 3-kinase/Akt- and MEK1/ERK-dependent pathways." dans: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19922-8, (2002) (PubMed).

Aplin, Stewart, Assoian, Juliano: "Integrin-mediated adhesion regulates ERK nuclear translocation and phosphorylation of Elk-1." dans: The Journal of cell biology, Vol. 153, Issue 2, pp. 273-82, (2001) (PubMed).

Robinson, Cheng, Khokhlatchev, Ebert, Ahn, Guan, Stein, Goldsmith, Cobb: "Contributions of the mitogen-activated protein (MAP) kinase backbone and phosphorylation loop to MEK specificity." dans: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29734-9, (1997) (PubMed).

Détails sur MEK1

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Autre désignation: MEK1

Sujet: MEK1 (MapK/ERK Kinase 1) is a 45-kDa member of the MEK family of dual specificity kinases. MEK is activated by a variety of cellular serine/threonine kinases including c-Raf, A-Raf, c-mos, and MEK Kinase-1. Activated MEK phosphorylates MAP kinase (ERK) at threonine and tyrosine residues. This results in activation of ERK and its signaling pathway. MEK is highly specific for ERK and various MEKs preferentially phosphorylate individual ERK isoforms. MEK1 only activates ERK1 and ERK2. This specificity may result from variations in ERK regions that are known as the phosphorylation lip and kinase backbone. MEK's localization is cytoplasmic, but mitogenic stimulation induces a mass translocation to the nucleus. Mechanisms behind this nuclear translocation remain unknown. However, MEK contains an N-terminal nuclear export signal (NES) that mediates its rapid exodus from the nucleus and restores its unstimulated cellular distribution. The 25/MEK1 monoclonal antibody recognizes MEK1, regardless of phosphorylation status. The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure). Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.
Synonyms: MAPK/ERK kinase 1, EC 2.7.12.2, kinase MEK1, MAPKK1, PRKMK1

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling